I have limited information on EGB. According to the book "The Biles-Byles Family" he had 3 children of his own. (F.H., A.D., and Ellen Biles)

He is listed as one out of eight children born to Charles Biles (b.1776 m. Jane Miles, worked as surveyor near Pittsburg, PA)

Siblings listed: Jehial, Eleanor, Mary, John, Francis Asbury (b.11-27-1821 d. Lancaster, MO 8-22-1897, m. Nancy Bass in IN 11-13-1824), Andrew Jackson, William Patterson (b. 1814)

Children of siblings list Indiana and Schuyler County MO as birth locations.

The only closely related Alfred Biles I show is the son of Francis Asbury Biles. Alfred Graham Biles (b. 11-27-1847, d. 1-3-1925, m. Spicey Cardelia Jane Tipton) had a daughter named Clara Esabell who was born in Schuyler County, Missouri.

Hope this helps.