Jacob and Elizabeth Mlsna arrived
at New Orleans on 13 Dec 1860
on Ship Anna Delius from Bremen, Germany
See attached image of ship passenger list page
Last name misspelled as "Milsna"
I located this record after finding Jacob Mlsna's 12 Mar 1866 Declaration of Intention to become a US citizen in La Crosse County naturalization records (see record in upper left corner of the image at:https://familysearch.org/pal:/MM9.3.1/TH-1951-29966-2065-0?c...
The Declaration of Intention recorded that he arrived at New Orleans in Dec 1860 and with that info I was able to find the attached arrival record.
Jacob was issued a federal land patent for 40 acres in the town of Washington, La Crosse County in 1863 (Township: 15-N, Range: 5-W, NW qtr of NE qtr of Section 33 -- that would be east of what is now County Rd PI and about a mile north of the county line).
Jacob and Elizabeth were in Hamburg, Vernon County at the 1870 census with five children (Hamburg is adjacent to and south of Washington, La Crosse County) . Their last name is misspelled as Misner. Elisabeth's parents, Mathias (aka, Matej) and Mary (aka, Maria) Nekola (last name misspelled as Nicolai) were also in the household.
Burial records for the Mlsna and Nekola families are available in La Crosse Library genealogy indexes at http://www.lacrosselibrary.org/genealogy/historicevents.asp
Look there for Matej Nekola, Maria Nekola, Jacob Mlsna, and Elizabeth Mlsna. (Make sure the "Cemeteries" option is checked instead of the Obituaries option.)
Jacob and Elizabeth were recorded on page 20 of the 1880 census at Hamburg, Vernon County, with nine children. The name appears to be written as "Mesna" on the census page (Ancestry.com mangled it as "Meonie" in their index).
The registration of Jacob's "final papers" for citizenship in 1872 recorded his last name as "Mazna" which I think is a Germanized spelling of the name.
That is the spelling of his last name in the record for his marriage to Elizabeth:
Mazna, Jakob (farm laborer of Luznice) and Nekola, Marie of Spoli #24, married 15 Feb 1859 in Trebon parish. Jacob's parents are named in the marriage record as "Jana Mazny" (nominative case would be: Jan Mazna) and "Marie Spevak". http://digi.ceskearchivy.cz/en/8049/281
The 1831 baptism record at Luznice in Trebon parish for "Jacobus Mazna" (born 23 Nov 1831, baptized the next day) son of "Johann Mazna" (Jan Mlsna) and "Maria Spiwak" (Marie Spevak) is at http://digi.ceskearchivy.cz/en/8032/236